Raman Molecular Imaging Analysis
of Cellular Apoptosis
Raman Chemical Image Overlay
Currently available methods to detect and evaluate normal and abnormal cell death, apoptosis, depend on sophisticated reagents that mark the presence or absence of specific molecules in the cells. Though readily available, these assays are often challenging to interpret. Raman molecular analysis for apoptosis is a sensitive, reagentless technique, making it an alternative to traditional fluorescence-based cell death assays.
Why Use Raman Molecular Imaging For Apoptosis Analysis?
- Completely reagentless
- No multi-step cellular assay needed
- Measure biologically relevant changes in cellular systems
- Easy identification of specific molecular and physiological information about cells
Raman molecular images are acquired from cell samples illuminated by a laser in a microscope. The images and spectral information are analyzed using chemometric-based classification algorithms to objectively classify the samples in terms of apoptotic state. Raman chemical imaging takes the reagents
out of the interpretive equation,
in other words, and yields specific molecular information about
samples under study.