Raman Molecular Imaging For Digital Pathology
H&E Stained Prostate Tissue
Raman Image of Unstained Prostate Tissue
Performed in collaboration with the Mayo Clinic and Allegheny General Hospital
Accurate interpretation of pathology specimens can be very challenging for a number of tissue and disease types. Traditional pathological evaluation of tissues and cells is a relatively subjective evaluation of spatially complex stained tissue samples. Since a physician makes treatment decisions based on the evaluation of tissue by a pathologist, accuracy is of the utmost importance. ChemImage’s Raman Molecular Imaging (RMI) approach using the FALCON II™
enables the objective assessment of tissues without the use of stains or reagents.
Raman Molecular Imaging Gives Pathologists:
- Molecular specificity
- Sensitivity to subtle molecular differences
- Measurement of Raman signals from the molecular constituents of a sample without reagents
- Measurements that are spatially localized within a given sample
Raman molecular images are acquired from tissue samples illuminated by a laser in a microscope. The images are analyzed using chemometric-based classification algorithms to objectively classify the sample in terms of disease state. RMI is used to create, in effect, a digital stain of tissues and cells—without the use of reagents.