Fluorescence Imaging of Biological Samples


Quantum dot labeled
Western Blot

Digital Image of Quantum Dot
Digital Image
 
Fluorescence Image of Quantum Dot
Fluorescence Image

Samples courtesy of Crystalplex

When a user wants to perform more than one assay on the same cells or tissue, multiple expensive fluorescent probes and filter sets are required. However, the overlapping fluorescence spectrum of many probes precludes the use of multiple dyes in routine use. Fluorescence imaging of biological samples using tunable filter systems helps the pathologist overcome the challenge of using multiple dyes for greater efficiency without sacrificing accuracy.

Fluorescence Imaging Of
A Biological Sample:

  • Uses tunable filter systems to permit the use of multiple dyes
  • Allows the study of multiple dyes without changing hardware
  • Permits separation of multiple overlapping dyes while reducing autofluorescence
  • Offers a chemometric-based analytical approach

Fluorescence spectral images are acquired from fluorescent samples illuminated by an appropriate light source. Images are taken with either a microscope or a stand-off macroscope. The images are analyzed using chemometric-based approaches to answer questions related to the biological samples.