Fluorescence Imaging of Biological Samples

When a user wants to perform more than one assay on the same cells or tissue, multiple expensive fluorescent probes
and filter sets are required. The overlapping fluorescence spectrum of many probes precludes the use of multiple dyes
in routine use.

Tunable filter systems are used to study multiple dyes, without the requirement of changing hardware. Chemometric
analysis enables separation of multiple overlapping dyes and reduction of autofluorescence.

Fluorescence spectral images are acquired from fluorescent samples illuminated by an appropriate light source and taking images with either a microscope or a stand-off macroscope. The images are analyzed using chemometric based approaches to answer questions related to the samples.

Want to know more? Download the proceedings document:
"Nanocrystal Clusters in Combination with Spectral Imaging to Improve Sensitivity in Antibody Labeling Applications of Fluorescent Nanocrystals"

Standard Photograph of Gel (Figure 1) and Chemical Image
of Nanocluster Labeled (Green) Gel (Figure 2)

*Samples provided courtesy of Crystalplex

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