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Figure 1. (Top) Brightfield transmission / NIR Chemical fusion image reveal specific oil droplets in the emulsion. (Bottom) Droplet size distribution histogram |
Wide-Field Chemical Imaging which combines spectroscopy with optical microscopy is a rapid, noninvasive technique that details sample morphology, composition and spatial distribution. Advantages in speed and image clarity favor Chemical Imaging over more conventional microspectroscopy. In this example, NIR chemical imaging complements the Raman imaging capabilities of the FALCON II™ Wide-Field Raman Chemical Imaging System.
- Determines or confirms the droplet size of oils in emulsions
- Simultaneously detects droplets of different chemical compositions
- Optional NIR imaging complements standard FALCON II
The FALCON II uses the molecular signature of the components to create images of the emulsion with chemical contrast. The droplets are sized and counted using ChemImage Xpert™ software. A fusion of the brightfield optical image with the false-colored NIR chemical image exposes spatial distribution of oil droplets. Figure 1 illustrates the emulsion droplet fusion image as well as a histogram of the droplet size distribution.
A variety of geometric parameters are available for analysis. Experiments can be automated to collect multiple fields of view to achieve an improved statistical measure of droplet size distribution. The specificity of NIR Chemical Imaging may be employed to obtain the size distribution of multiple components simultaneously.
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