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Our customers participate in a thorough three-day training program using our imaging technology to certify they understand how to best acquire and analyze information for their chemical and biomedical analytical applications.This three-day course includes both theoretical and practical training. Each participant will be given time on a FALCON II Chemical Imaging System to acquire several Chemical Imaging datasets and analyze them. After the finishing the training course, each participant should be able to collect and analyze data for simple systems.
Day 1: Introduction to Chemical Imaging Techniques
ChemImage Instrumentation Overview
Day 2: Introduction to Data Acquisition Techniques
Introduction to Data Analysis
Day 3: Introduction to Chemometrics
Application Development Techniques
Day 1 AM |
Introduction to Chemical Imaging Techniques
- Overview Presentation
- Determine Objectives for Your Training
- Introduction to Spectroscopy Fundamentals
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Day 1 PM |
ChemImage Instrumentation Overview
- Instrument Start up
- Becoming familiar with the Falcon
- Microscope
- Turrets
- Laser
- Selecting Correct Configuration
- Digital Images
- Brightfield Reflectance
- Brightfield Transmittance
- Polarized Light Microscopy
- Differential Interference Contrast
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Day 2 AM |
Introduction to Data Acquisition Techniques
- How to Calibrate the Spectrometer
- Acquiring Sample Spectrum
- Sample Preparation
- Trouble shooting with three tiers of materials
- Good Raman scatterers
- Weak Raman scatterers
- Highly fluorescent materials
- Photobleaching
- Acquiring and Applying Corrections
- Timed Spectral Acquisitions
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Day 2 PM |
Introduction to Data Analysis
- Cosmic Filter
- Baseline Correction
- Vector Normalization
- Construction – Build, Crop, extract
- RGB Generation – Composite, Stretch, Overlay
- Statistics – ROI Statistics, Particle Statistics
- Filters – Gaussian Blur, Morphological, Reduce Noise
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Day 3 AM |
Introduction to Chemometrics
- Principle Component Analysis
- Spectral Mixture Resolution
- Partial Least Squares Regression
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Day 3 PM |
Application Development Techniques
- Customer specific sample analysis
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